Purification and identification of HMGB1 secreted by liver cells and immune cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 1065-1070, 2011.
Article
in Zh
| WPRIM
| ID: wpr-814475
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To purify and identify HMGB1 secreted by liver cells HepG2 and immune cells U937.@*METHODS@#We cultured the liver cell lines HepG2 and immune cell lines U937, and stimulated them with HMGB1 (400 ng/mL) for 20 h. Then the supernatant was collected. Ultrafiltration centrifugation, CM-Sepharose cation, DEAE-Sepharose anion exchange chromatography, Sephadex G75-gel filtration chromatography, and immunoprecipitation were used for purification. The molecular weight and identity of HMGB1 was confirmed by SDS-PAGE and Western blot.@*RESULTS@#A sharp stained protein band with a molecular weight of about 26 kD was obtained by SDS-PAGE analysis and shown to be HMGB1 confirmed by Western blot.@*CONCLUSION@#High purified HMGB1 can be separated from these two cell lines.
Full text:
1
Index:
WPRIM
Main subject:
Monocytes
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Cell Culture Techniques
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U937 Cells
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Hepatocytes
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HMGB1 Protein
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Electrophoresis, Polyacrylamide Gel
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Hep G2 Cells
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Metabolism
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Methods
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Year:
2011
Type:
Article