Effect of high glucose peritoneal dialysis solution on PGC-1α expression and mitochondria related oxidative injury in human peritoneal mesothelial cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 1085-1091, 2013.
Article
in Zh
| WPRIM
| ID: wpr-814807
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the mechanism of mitochondrial oxidative injury induced by high glucose peritoneal dialysis solution (PDS) and the protective effect of peroxisome proliferator activated receptor gamma coactivator 1-alpha (PGC-1α) in the mitochondria of human peritoneal mesothelial cells (HPMC) in the high glucose ambience.@*METHODS@#HPMC was cultured in a PDS containing 1.5%, 2.5% and 4.25% glucose for 24 hours. Western blot analysis was used to detect PGC-1α expression. MitoSOX? Red staining, respiratory chain complexes and antioxidant enzyme activities were determined.@*RESULTS@#The activities of respiratory chain complex III and antioxidant enzymes decreased significantly in a concentration- and time-dependent manner, along with the increased production of mitochondrial reactive oxygen species (ROS) and cellular apoptosis. In addition, protein expression of PGC-1α was also decreased in the high glucose PDS ambience.@*CONCLUSION@#High glucose PDS might inhibit PGC-1α expression, resulting in the inhibition of mitochondrial function and increase of mitochondrial ROS and cellular apoptosis.
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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Transcription Factors
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Dialysis Solutions
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Peritoneal Dialysis
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Reactive Oxygen Species
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Apoptosis
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Oxidative Stress
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Epithelial Cells
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Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
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Glucose
Limits:
Humans
Language:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Year:
2013
Type:
Article