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Role of nerve growth factor in differentiation of 
dendritic cells / 中南大学学报(医学版)
Zhongnan Daxue xuebao. Yixue ban ; (12): 829-836, 2015.
Article in Zh | WPRIM | ID: wpr-815262
Responsible library: WPRO
ABSTRACT
OBJECTIVE@#To explore the effect of nerve growth factor (NGF) on the  differentiation of murine bone marrow-derived dendritic cells (DCs) in vitro.
@*METHODS@#The bone marrow cells of femur and tibia from healthy C57B -L/6 mice were isolated and divided into 4 groups: a phosphate buffered saline (PBS) group (PBS group), a NGF group, a granulocyte monocyte colony stimulating factor (GM-CSF) plus interleukin 4 (IL-4) group (GM-CSF+IL-4 group), and a GM-CSF plus IL-4 and NGF group (n=6 in each group). The positive rate of CD11c+ and the proportion of CD8a- were compared at the 7th day among the different groups by flow cytometry. The immature DCs were acquired by classic methods with GM-CSF and IL-4. The purified DCs were obtained by magnetic bead positive selection for CD11c+ cells. The immature DCs were divided into 4 groups: a PBS group, a NGF group, a LPS group, and a NGF+LPS group (n=6 in each group), which were incubated with PBS, NGF, LPS and NGF+LPS, respectively. Cytokine levels of IL-6, IL-10 and IL-12 were detected by ELISA after 24 hours..
@*RESULTS@#1) the percentage of CD11c+ DCs in the NGF group were more than that in the PBS group, and lower than that in the the GM- CSF+IL-4 group (both P0.05). CD8a- DCs were dominant in these four groups; 2) NGF could further up-regulate the LPS-induced cytokine secretion from DCs, such as IL-6, IL-10, and IL-12 (all P<0.05), but NGF alone had no such effect (all P<0.05).
@*CONCLUSION@#NGF can promote the murine bone-marrow cells differentiation into CD11c+ DCs, with CD8a-subset; NGF could enhance LPS-induced cytokine secretion from DCs (IL-6, IL-10 and IL-12).
Subject(s)
Full text: 1 Index: WPRIM Main subject: Pharmacology / Dendritic Cells / Bone Marrow Cells / Cell Differentiation / Cells, Cultured / Granulocyte-Macrophage Colony-Stimulating Factor / Interleukin-4 / Interleukin-6 / Interleukin-10 / Interleukin-12 Limits: Animals Language: Zh Journal: Zhongnan Daxue xuebao. Yixue ban Year: 2015 Type: Article
Full text: 1 Index: WPRIM Main subject: Pharmacology / Dendritic Cells / Bone Marrow Cells / Cell Differentiation / Cells, Cultured / Granulocyte-Macrophage Colony-Stimulating Factor / Interleukin-4 / Interleukin-6 / Interleukin-10 / Interleukin-12 Limits: Animals Language: Zh Journal: Zhongnan Daxue xuebao. Yixue ban Year: 2015 Type: Article