Simultaneous Determination of 7 Anti-rheumatic Active Ingredients in Mongolian Medicine Sendeng- 4 Decoction Powder by HPLC-DAD / 中国药房
China Pharmacy
; (12): 221-225, 2019.
Article
in Zh
| WPRIM
| ID: wpr-816725
Responsible library:
WPRO
ABSTRACT
OBJECTIVE: To establish a method for simultaneous determination of 7 kinds of anti-rheumatic active ingredients in Mongolian medicine Sendeng-4 decoction powder, such as catechin, jasminoidin, dihydromyricetin, texifolin, rutin, myricetin and quercetin. METHODS: HPLC-DAD method was adopted. The determination was performed on Sil Green C18 column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solution (gradient elution) at the flow rate of 1 mL/min. The detection wavelength was set at 238 nm, and column temperature was 30 ℃. The sample size was 10 μL. RESULTS: The linear range of catechin, jasminoidin, dihydromyricetin, texifolin, rutin, myricetin and quercetin were 8.590-2 290, 10.56-3 901, 13.00-3 958, 8.815-564.2, 4.030-257.8, 8.130-750.5, 7.075-454.2 μg/mL (r≥0.999 1), respectively; the limits of detection were 0.429 5, 0.264 0, 0.325 0, 0.220 4, 0.201 5, 0.203 2, 0.176 9 μg/mL, respectively; the limits of quantification were 1.030, 1.321, 1.302, 1.397, 1.637, 0.813 0, 0.707 5 μg/mL, respectively. RSDs of precision, stability (48 h) and repetition tests were all lower than 2.0% (n=6). The average recoveries were 96.24%-99.28% (RSD=1.03%-1.63%, n=6). CONCLUSIONS: The established method is simple, accurate and reproducible, and can be used for simultaneous determination of catechin, jasminoidin, dihydromyricetin, texifolin, rutin, myricetin and quercetin in Mongolian medicine Sendeng-4 decoction powder.
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Index:
WPRIM
Language:
Zh
Journal:
China Pharmacy
Year:
2019
Type:
Article