Protective effect of DNA vaccine with the gene encoding 55kDa antigen fragment against Pneumocystis carinii in mice
Asian Pacific Journal of Tropical Medicine
; (12): 353-356, 2011.
Article
in En
| WPRIM
| ID: wpr-819507
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To evaluate the protective effect of DNA vaccine with the gene encoding 55kDa antigen fragment of Pneumocystis carinii (P. carinii) against P. carinii in mice.@*METHODS@#The fragment of the antigen within p55(p55-582) was cloned. Then recombinant plasmid was constructed based on the eukaryotic expression vector pcDNA3.1(+). BALB/c mice were used as experimental models to examine the immunogenicity of pcDNA3.1(+)-p55-582. ELISA and RT-PCR were used to evaluate the role of this kind of DNA vaccine.@*RESULTS@#The results of western blot indicated that the recombinant DNA[pcDNA3.1(+)-p55-582] could be expressed correctly and had antigenicity in transfected COS-7 cells. ELISA and RT-PCR showed that pcDNA3.1(+)-p55-582 elicited antibody production, stimulated lymphocyte proliferation and provided partial protection by reducing the P. carinii burden.@*CONCLUSIONS@#The data demonstrate that pcDNA3.1(+)-p55-582 might be potent vaccination that can afford the partial protection for the immunized animals.
Full text:
1
Index:
WPRIM
Main subject:
Enzyme-Linked Immunosorbent Assay
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Viral Vaccines
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Chlorocebus aethiops
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Immunization
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COS Cells
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Vaccines, DNA
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Pneumocystis carinii
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Allergy and Immunology
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Real-Time Polymerase Chain Reaction
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Genetics
Limits:
Animals
Language:
En
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2011
Type:
Article