Optimization of a fluorescent qPCR detection for RNA of SARS-CoV-2 / 生物工程学报
Chinese Journal of Biotechnology
; (12): 732-739, 2020.
Article
in Zh
| WPRIM
| ID: wpr-826903
Responsible library:
WPRO
ABSTRACT
We optimized a fluorescent quantitative polymerase chain reaction (qPCR) assay system for rapid and real time detection of SARS-CoV-2 RNA. The results show that the lowest dilution of RNA samples used for the detection of SARS-CoV-2 RNA could reach 1/10 000 (the initial value is set as 10 ng/μL). Moreover, the cycle threshold (Ct) for samples of clinically diagnosed COVID-19 was lower than 35 or 40. The sensitivity of this method was satisfactory. The results were consistent with those of the COVID-19 detection kit on the market under the same conditions, but the number of cycles required was shortened by about 2. Therefore, the optimized assay developed in this study can be used in screening and early clinical diagnosis. Our work provides a tool to facilitate rapid clinical diagnosis of COVID-19.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Pneumonia, Viral
/
Reference Standards
/
Time Factors
/
Virology
/
RNA, Viral
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
Coronavirus Infections
/
Early Diagnosis
/
Diagnosis
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2020
Type:
Article