Proteomics and peptidomics analysis of Sepiae Endoconcha / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3883-3889, 2020.
Article
in Zh
| WPRIM
| ID: wpr-828371
Responsible library:
WPRO
ABSTRACT
Shotgun based proteomics and peptidomics analysis were used to investigate the proteins and peptides in marine traditional Chinese medicine(TCM) Sepiae Endoconcha(cuttlebone). Peptides were extracted from cuttlebone by acidified methanol, and then strong cation exchange(SCX) resin was used to enrich those peptides. Also, proteins from cuttlebone were extracted and digested by trypsin. nano-LC Q Exactive Orbitrap mass spectrometry was used to analyze proteins and peptides from cuttlebone. As a result, a total of 16 proteins and 168 peptides were identified by protein database search, and 328 peptides were identified by De novo sequencing. The identified proteins were hemocyanin, enolase, myosin, actin, calmodulin, etc., and the identified peptides were derived from actin, histone, and tubulin. All these proteins and peptides were important components in cuttlebone, which would provide important theoretical and research basis for marine TCM cuttlebone investigations.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Peptides
/
Mass Spectrometry
/
Cations
/
Databases, Protein
/
Proteomics
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2020
Type:
Article