Characteristics of Atmospheric Fine Particulate Matter (PM ) Induced Differentially Expressed Proteins Determined by Proteomics and Bioinformatics Analyses / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 583-592, 2020.
Article
in En
| WPRIM
| ID: wpr-828976
Responsible library:
WPRO
ABSTRACT
Objective@#To screen the differentially expressed proteins (DEPs) in human bronchial epithelial cells (HBE) treated with atmospheric fine particulate matter (PM ).@*Methods@#HBE cells were treated with PM samples from Shenzhen and Taiyuan for 24 h. To detect overall protein expression, the Q Exactive mass spectrometer was used. Gene ontology (GO), Kyoto encyclopedia of genes and genomes (KEGG), and Perseus software were used to screen DEPs.@*Results@#Overall, 67 DEPs were screened in the Shenzhen sample-treated group, of which 46 were upregulated and 21 were downregulated. In total, 252 DEPs were screened in the Taiyuan sample-treated group, of which 134 were upregulated and 118 were downregulated. KEGG analysis demonstrated that DEPs were mainly enriched in ubiquitin-mediated proteolysis and HIF-1 signal pathways in Shenzhen PM samples-treated group. The GO analysis demonstrated that Shenzhen sample-induced DEPs were mainly involved in the biological process for absorption of various metal ions and cell components. The Taiyuan PM -induced DEPs were mainly involved in biological processes of protein aggregation regulation and molecular function of oxidase activity. Additionally, three important DEPs, including ANXA2, DIABLO, and AIMP1, were screened.@*Conclusion@#Our findings provide a valuable basis for further evaluation of PM -associated carcinogenesis.
Key words
Full text:
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Index:
WPRIM
Main subject:
Particle Size
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Mass Spectrometry
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Bronchi
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Gene Expression
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Computational Biology
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Proteomics
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Air Pollutants
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Epithelial Cells
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Particulate Matter
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Metabolism
Limits:
Humans
Language:
En
Journal:
Biomedical and Environmental Sciences
Year:
2020
Type:
Article