Transplantation of autologous angiogenin-overexpressing marrow stromal cells in treatment of a porcine chronic ischemia model / 第二军医大学学报
Academic Journal of Second Military Medical University
; (12): 586-591, 2014.
Article
in Zh
| WPRIM
| ID: wpr-839151
Responsible library:
WPRO
ABSTRACT
Objective: To investigate the effect of autologous angiogenin(ANG)-overexpressing marrow stromal cells transplantation on the neoangiogenesis and cardiac function in a porcine model of chronic ischemia. Methods: Porcine models of chronic ischemia were established and were randomized into the following 3 groups: autologous marrow stromal cells transfected with Ad-ANG (Group I, n=11), autologous marrow stromal cells without transfection (Group II, n=10), and controls injected with serum-free DMEM medium (Group III, n=10). The autologous marrow stromal cells were cultured and were transfected with Ad-ANG, and the cells were labeled by CM-Dil. The cels were transplanted to the ischemia site of porcine heart. The parameters we observed included Rentrop score, ejection fraction, percentage of infracted area, CM-Dil labeled cells under fluorescence microscope, number of blood vessels and expression of ANG protein. Results: Compared with group H and IE, Group I had significantly increased Rentrop score, ejection fraction, numbers of blood vessels in the infarcted areas, CM-DiI labeled cells, and ANG protein expression, but significantly decreased infarcted area (P < 0. 05 or P < 0. 01). Conclusion: Transplantation of autologous angiogenin-overexpressing marrow stromal cells can yield a high survival rate of cells, greatly improving the blood supply of the infarcted cardiac area and cardiac function in porcine model.
Full text:
1
Index:
WPRIM
Type of study:
Clinical_trials
/
Prognostic_studies
Language:
Zh
Journal:
Academic Journal of Second Military Medical University
Year:
2014
Type:
Article