Transplantation of marrow mesenchymal stem cells through renal artery in repair of acute tubular necrosis in nude mice / 第二军医大学学报

ABSTRACT

Objective: To investigate whether transplantation of mesenchymal stem cells(MSCs) through renal arteries can protect kidney from acute tubular necrosis(ATN), so as to lay a foundation for MSC transplantation in treatment of ATN. Methods: Five-week-old nude mice were randomly divided into three groups normal control group (n = 10), acute tubular necrosis(ATN) model group without(n = 10) and with MSCs treatment group(n = 11). ATN nude mice were induced with 50% glycerin. MSCs labeled with enhanced green fluorescent proteins(EGFP) were injected into kidney through renal arteries. Serum creatinine was determined in all groups and pathological changes of renal tissues were detected using H-E staining. The amount and distribution of the EGFP-marked MSCs in renal tissues were determined with fluorescence microscope. Results: Degeneration and exfoliation of renal tubular epithelial cells, and even renal tubular tamponade with cast-off cells were observed in the ATN group; these pathological changes were mainly located at renal cortex and juncture of renal cortex and medulla. The damages were greatly alleviated in the ATN + MSCs transplantation group, with no swelling of epithelial cells, nuclear condensation or edema. Fourteen days after MSCs transplantation, EGFP positive cells were increased in renal tubules of recipient mice. Conclusion: The MSCs transplantation via renal artery can locate in renal tubular epithelium, and promote the repair of injured renal tubular epithelial cells.