Cytotoxic effects of granulysin on Candida albicans / 第二军医大学学报

ABSTRACT

Objective: To study the cytotoxic effects of granulysin on Candida albicans. Methods: Candida albicans were cultured with different concen trations of granulysin peptides and the colonies of Candida albicans on Sabouraud dextrose agar were calculated. Broth microdilution method was used to determine the minimum inhibitory concentrations (MIC) of granulysin and fluconazole on Candida albicans. Results: When granulysin peptides 2 and 3 (corresponding to G2 and G3 peptides) were at 20 μg/ml, the average colonies of Candida albicans decreased from 838 and 927 to 203 and 218, respectively; G1, G4 and G5 did not reduce the average colony of Candida albicans even at 40 μg/ml. Three strains of Candida albicans were sensitive to granulysin, with their MICs being 26, 22, 29 μg/ml, and their MICs to fluconazole were 4, 20 and 128 μg/ml. Conclusion: Granulysin has cytotoxic effects on Candida albicans and is one of the natural anti-fungi proteins in human body; it has a promising future for anti-fungi drug development.