Primary cutaneous cryptococcosis in mice: An experimental study / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 143-145, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-841491
ABSTRACT
Objective:
To establish an animal model of primary cutaneous cryptococcosis and to study the role of immune suppression in Cryptococcus neoformans (C. neoformans) infection.Methods:
Twenty-four BALB/c mice were equally randomized into 2 groups immunocompetent group and immunocompromised group. C. neoformans isolate, B3501, was intradermaly inoculated at a concentration of 5 × 107/ml into all mice. Mycology and histopathology examinations of the skin were done to confirm the infection. The skin damage courses were recorded and compared in 2 groups.Results:
Different manifestations of skin infection, such as nodule, papule, ulcer and lesions mimicking molluscum contagiosum appeared 2-9 d after inoculation, with a mean of (3.42±1.17) d in immunocompromised group and (4.25±1.42) d in immunocompetent group. The lesions became self-cured 22-44 d after their onset, with a mean of (36.75±4.20) d in immunocompromised group and (29.00±4.75) d in immunocompetent group (P<0.05). Cryptococcal infection was confirmed by mycology culture and histopathology examination.Conclusion:
Intradermal inoculation with C. neoformans is feasible for establishing a cutaneous cryptococcosis model in BALB/c mice. Immnunosupressent state may not be a key factor for primary cutaneous cryptococcosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Controlled clinical trial
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2006
Type:
Article
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