UGT88B2: A promiscuous O-glycosyltransferase from Carthamus tinctorius / 中草药·英文版
Chinese Herbal Medicines
; (4): 440-445, 2020.
Article
in Zh
| WPRIM
| ID: wpr-841996
Responsible library:
WPRO
ABSTRACT
Objective:
In order to obtain new glycosyltransferases with highly efficient catalysis, the glycosyltransferases from Carthamus tinctorius which contains diverse types of glycosides were mined.Methods:
A new glycosyltransferase gene (UGT88B2) with full length was obtained by PCR and further transformed into Escherichia coli for heterologous expression. The catalytic activity of recombinant UGT88B2 was determined by HPLC-MSn. The structures of representative catalytic products were elucidated by MS and NMR.Results:
UGT88B2 exhibited catalytic promiscuity and various patterns in glycosylation of flavonoids with high efficiency.Conclusion:
A new glycosyltransferase named UGT88B2 was successfully mined and can be employed as enzymatic tools in glycosylation of flavonoids.
Full text:
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Herbal Medicines
Year:
2020
Type:
Article