Study on the Protective Effects of Butein on Oxidative Stress Injury of PC 12 Cell and Its Effects on Mitochondrial Function / 中国药房
China Pharmacy
; (12): 2974-2981, 2020.
Article
in Zh
| WPRIM
| ID: wpr-843075
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:To study the protective effects of butein on oxidative stress injury of PC12 cell and its effects on mitochondrial function. METHODS:Rats PC12 cells were divided into normal control group,model group,solvent control group(1 ‰ dimethyl sulfoxide),butein high,medium and low concentration groups(2,1,0.5 μmol/L). The latter 4 groups were given relevant reagent/medicine for intervention;24 h later,other groups were given 100 mU/mL glucose oxidase to induce oxidant stress model except for normal control group. After 4 h culture,cell survival rate,apoptosis rate,the levels or activities of ROS,MDA,SOD,CAT,GSH-Px,ATP,IL-1β and TNF-α as well as the change of MMP were detected. RESULTS:Compared with normal control group,cell survival rate,the levels or activities of SOD,CAT,GSH-Px and ATP were all decreased significantly,and apoptotic rate,the content of ROS,the levels of MDA,IL-1β and TNF-α were all increased significantly(P<0.05 or P<0.01),while the MMP was decreased significantly. Compared with model group,above indexes of solvent control group had no significant change (P>0.05),cell survival rates,the levels or activities of SOD (except for medium and low concentration groups),CAT,GSH-Px(except for medium and low concentration groups),ATP(except for low concentration group)were increased significantly in butein high,medium and low concentration groups,while apoptotic rates,the content of ROS,the levels of MDA,IL-1 β and TNF-α were decreased significantly(P<0.05 or P<0.01),while the MMP were increased significantly. CONCLUSIONS:Butein can increase the antioxidant enzyme activity, stabilize mitochondrial function, inhibit oxidative stress and inflammationthus, increase energy generation inhibiting neuronal cell apoptosis ultimately exerting a neuroprotective effect.
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Index:
WPRIM
Language:
Zh
Journal:
China Pharmacy
Year:
2020
Type:
Article