A new method for primary culture of mouse dorsal root ganglion neurons / 西安交通大学学报(医学版)

ABSTRACT

Objective: To establish a simple method for primary culture of mouse dorsal root ganglion neurons of high purity. Methods: The dorsal root ganglions from healthy C57BL/6 mice of 6-8 weeks were taken to obtain dorsal root ganglion neurons by using type collagenase and trypsin digestion. Identification and purification were evaluated by neuron specific enolase (NSE) monoclonal antibody immunocytochemistry staining. Results: The cultured primary neurons grew well and the purity could reach about 90%. The survival time was 60 days when cultured with the DMEM medium containing nerve growth factor (NGF). Conclusion: The culture program is simple and stable, and can cultivate a large number of high-purity neurons, which provides a reliable model for in-depth study of neurons.