Effect of porcine kidney epithelial cells type I interferon receptor 1 knockdown on Pseudorabies virus replication / 解剖学报
Acta Anatomica Sinica
;
(6): 741-746, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-844574
ABSTRACT
Objective:
To investigate the effect of type I interferon receptor 1 (IFNAR1) on Pseudorabies virus (PRV) replication.Methods:
A stable cell line in which porcine kidney epithelial cells (PK 15) knocked out the IFNAR1 gene was constructed using a lentiviral-mediated CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats, CRISPR) gene editing technique. The function of IFNAR1 was verified by cell viability assay, fluorescence observation, flow cytometry detection, titer determination, and Real-time PCR.Results:
With the prolongation of PRV infection, knocking out the IFNAR1 gene can significantly promote transcription of PRV-TK mRNA, translation of PRV-gE protein, and virulence of progeny virus.Conclusion:
IFNAR1 plays an important role in inhibiting the proliferation of PRV.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Anatomica Sinica
Year:
2019
Type:
Article
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