Preparation of asiaticoside sodium alginate repair patch and its wound healing effect / 中草药

ABSTRACT

Objective: Asiaticoside-sodium alginate repair patch (As-SA RP) was prepared and its effect on skin wound healing was investigated. Methods: Based on the cross-linking characteristics of sodium alginate and Ca2+, As-SA RP was prepared using asiaticoside as a drug model through blending with sodium alginate. The compatibility of the blend films was investigated by scanning electron microscope and FTIR. The whole layer of skin on both sides of the back spine of New Zealand's big ear rabbit was used to establish the trauma model. The repair effect of the external application of As-SA RP on skin wounds was investigated by observing the healing status and determining the expression levels of collagen (hydroxyproline as an index) and pro-inflammatory (TNF-α, IL-1β and IL-6) factors in the injured skin tissue. Results: Asiaticoside had good compatibility with sodium alginate and can be successfully prepared As-SA RP. Compared with other groups, the red swelling, bleeding, infection, and exudate on injured skin were significantly reduced, and the wound healing rate was also relatively increased after As-SA RP treatment. Moreover, the less inflammatory cells infiltration, more new capillaries and intact wound skin structure relatively tight and orderly arrangement of collagen fibers were observed in the As-SA RP group. On the other hand, the biochemical analysis showed that As-SA RP could effectively inhibit the high expression of TNF-α, IL-1β, IL-6 in the injured skin tissue and significantly increase the contents of hydroxyproline and collagen fiber. Conclusion: As-SA RP could improve the skin absorption of asiaticoside and show the better effect of skin wound healing than the active component asiaticoside.