MicroRNA-490-5p inhibits invasion and migration of human intrahepatic cholangiocarcinoma RBE cells / 肿瘤

ABSTRACT

Objective: To investigate the expression of microRNA-490-5p (miR-490-5p) in human intrahepatic cholangiocarcinoma tissues, and to study its impacts oninvasion and migration of intrahepatic cholangiocarcinoma RBE cells and the underlyingmolecular mechanism.Methods: The expression level of miR-490-5p in 5 pairs of intrahepatic cholangiocarcinomatissues and the adjacent normal tissues was detected by real-time fluorescent quantitativePCR. Intrahepatic cholangiocarcinoma RBE cells were transfected with miR-490-5p-mimicsand the negative control by LipofectAMINE 2000, respectively. Then the cell migration andinvasion abilities were explored by scratch wound healing assay and Transwell chamberassay, the proliferation of RBE cells were measured by CCK-8 assay, the colony formationability was detected by colony formation assay, the apoptosis rate was detected by FCMmethod. The target genes of miR-490-5p were predicted by online miRNA-targeted geneprediction software, and validated by Western blotting.Results: The expression level of miR-490-5p was significantly down-regulated in humanintrahepatic cholangiocarcinoma tissue as compared with the adjacent normal tissues (P 0.05), but the scratch healing, migrationand invasion abilities of RBE cells were significantly decreased (all P < 0.05). TargetScanwebsite predicted that the potential target gene of miR-490-5p was FBJ osteosarcomaoncogene (c -Fos ), and Western blotting verified that the expression of c-Fos was significantlydown-regulated in RBE cells transfected with miR-490-5p-mimics.Conclusion: MiR-490-5p is lowly expressed in human intrahepatic cholangiocarcinoma, andmay regulate the migration and invasion of intrahepatic cholangiocarcinoma RBE cells bydown-regulating the expression of c -Fos gene.