PIK3CD gene silencing inhibiting the proliferation and migration of gastric cancer HGC-27 cells in vitro / 肿瘤

ABSTRACT

Objective: To investigate the effects of phosphoinositide-3 kinase, catalytic subunit delta (PIK 3CD ) gene silencing on the proliferation and migration of human gastric cancer HGC-27 cells in vitro and its possible mechanism. Methods: The expression level of PIK3CD mRNA in HGC-27 cells was detected by real-time fluorescent quantitative PCR. HGC-27 cells were transfected with recombinant plasmid MSCV-PIK3CD-shRNA, then the expression silencing of PIK 3CD gene was verified by real-time fluorescent quantitative PCR and Western blotting. The proliferation and migration abilities of HGC-27 cells after transfection with MSCV-PIK3CD-shRNA were detected by cell count kit-8 (CCK-8) assay and Transwell chamber assay, respectively. The expressions of Akt signal pathwayrelated proteins in HGC-27 cells after transfection with MSCV-PIK3CD-shRNA were detected by Western blotting. Results: The expression level of PIK3CD mRNA in gastric cancer HGC-27 cells was higher than that in normal gastric mucosal GES-1 cells. After transfection with MSCV-PIK3CD-shRNA, the expression levels of PIK3CD mRNA and protein in HGC-27 cells with high expression of PIK 3CD gene were significantly decreased (P < 0.05), and the cell proliferation and invasive abilities were significantly reduced (P < 0.05). PIK 3CD gene silencing had no effect on the expression of Akt, but significantly inhibited the expression of phospho-Akt (p-Akt) (P < 0.05). Conclusion: PIK3CD may promote the proliferation and migration of gastric cancer HGC-27 cells in vitro by activating Akt pathway.