Allergenic Characterization of a Novel Allergen, Homologous to Chymotrypsin, from German Cockroach
Allergy, Asthma & Immunology Research
; : 283-289, 2015.
Article
in En
| WPRIM
| ID: wpr-85013
Responsible library:
WPRO
ABSTRACT
PURPOSE: Cockroach feces are known to be rich in IgE-reactive components. Various protease allergens were identified by proteomic analysis of German cockroach fecal extract in a previous study. In this study, we characterized a novel allergen, a chymotrypsin-like serine protease. METHODS: A cDNA sequence homologous to chymotrypsin was obtained by analysis of German cockroach expressed sequence tag (EST) clones. The recombinant chymotrypsins from the German cockroach and house dust mite (Der f 6) were expressed in Escherichia coli using the pEXP5NT/TOPO vector system, and their allergenicity was investigated by ELISA. RESULTS: The deduced amino acid sequence of German cockroach chymotrypsin showed 32.7 to 43.1% identity with mite group 3 (trypsin) and group 6 (chymotrypsin) allergens. Sera from 8 of 28 German cockroach allergy subjects (28.6%) showed IgE binding to the recombinant protein. IgE binding to the recombinant cockroach chymotrypsin was inhibited by house dust mite chymotrypsin Der f 6, while it minimally inhibited the German cockroach whole body extract. CONCLUSIONS: A novel allergen homologous to chymotrypsin was identified from the German cockroach and was cross-reactive with Der f 6.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Immunoglobulin E
/
Enzyme-Linked Immunosorbent Assay
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Allergens
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Chymotrypsin
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Amino Acid Sequence
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Clone Cells
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Sequence Homology
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Cockroaches
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DNA, Complementary
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Blattellidae
Language:
En
Journal:
Allergy, Asthma & Immunology Research
Year:
2015
Type:
Article