Clone and expression analysis of CtWD40 in Carthamus tinctorius / 中草药

ABSTRACT

Objective: To clone CtWD40 transcription factors (TFs) and analyze its expression level in different tissues of Carthamus tinctorius and relationship with the content of hydroxysafflor yellow A (HSYA). Methods: CtWD40 gene was obtained by cloning the WD40 candidate gene from transcriptome of C. tinctorius as reference. Its conserved domain, three-dimensional structure and phylogeny analysis were analyzed by bioinformatics methods. The CtWD40 expression pattern was also analyzed by qRT-PCR method, in the same time, HSYA content in different petals were analyzed by HPLC. Results: Gene sequence of CtWD40 was obtained and eight conserved WD domains were found in CtWD40 gene sequences. Phylogenetic analysis revealed that CtWD40 had a closed homology with WD40 from composite plants. Conclusion: The expression of CtWD40 gene was first increased and then decreased in petal tissues of different flowering stages.Pearson coefficient revealed significant correlation between CtWD40 expression and HSYA content in C. tinctorius petal.