Novel carbon dots derived from Dryopteridis Crassirhizomatis Rhizoma Carbonisatum and their hemostatic effect / 中草药

ABSTRACT

Objective: A new type of nanoparticles-carbon dots was identified from Dryopteridis Crassirhizomatis Rhizoma Carbonisatum (DCC) and investigated their hemostatic effect. Methods DCC was boiled, filtered, and dialyzed to separate and obtain a novel type of carbon dots named DCC-CDs. These DCC-CDs were characterized using transmission electron microscopy (TEM) and high-resolution TEM, as well as Fourier transform infrared, ultraviolet-visible, X-ray photoelectron spectroscopy, and HPLC. Then, the anti-hemorrhagic effects were evaluated by severed tails ad liver bleeding tests and the related hemostatic mechanisms of the obtained DCC-CDs were assessed by detecting blood coagulation and PLT quantity to discuss the hemostasis mechanism. Results The DCC-CDs separated from DCC were spherical, monodisperse, which had a narrow size distribution between 1 and 7 nm. In the animal experiment, DCC-CDs were revealed excellent hemostatic bioactivity, and significant decreased in the tail and liver bleeding time (P < 0.01). Additionally, the rats showed a profound increasing in fibrinogen (FIB) and platelets (PLT) after DCC-CDs treatment (P < 0.05). Conclusion These results demonstrated an explicit hemostatic effect of DCC-CDs, which offers new avenues for research into DCC to treat hemorrhagic diseases and a new mindset for the exploration of the active ingredients of traditional Chinese medicine.