Bioinformatics analysis and fluorescence quantitative PCR results of Ricinus communis PIP5Ks / 中草药

ABSTRACT

Objective To predict the expression of seven genes in the PIP5K gene family, and to determine the relative expression of PIP5Ks in different stages of type Lm gynoecious Ricinus communis aLmAB2. Method Bioinformatics analysis of online software protein physicochemical properties analysis, protein hydrophobicity analysis subcellular localization prediction, transmembrane region analysis, etc. Bioinformatics analysis of hemp PIP5K gene family and DNAMAN for sequence alignment and real-time PCR. Results The results showed that there were seven members in the R. communis PIP5Ks, which were PIP5K1, PIP5K2, PIP5K4, PIP5K6, PIP5K8, PIP5K9, and PIP5K11. The amino acid sequence homology of the corresponding protein of R. communis PIP5K reached 48.06%, and the protein of R. communis PIP5Ks all are hydrophilic proteins. Except that PIP5K9 and PIP5K11 were unstable proteins, the rest were stable proteins, and the corresponding proteins of PIP5Ks have no transmembrane domain, and all were non-transmembrane proteins. Subcellular localization results showed that the proteins PIP5K1, PIP5K4, PIP5K6, PIP5K9, and PIP5K11 were less likely to have a lead peptide, and there was no corresponding amino acid cleavage site, ie, localized in other organelles or possibly cytoplasmic proteins; Protein PIP5K2 was localized in chloroplasts, the chloroplast transit peptide has a higher value, and the protein PIP5K8 was located in the secretory pathway. From the perspective of the relative expression of PIP5Ks in the differential expression, except that no fluorescence was detected in PIP5K4, the other PIP5Ks had a certain differential expression. The changes of relative expression of PIP5Ks showed similar trends, the levels of PIP5K1, PIP5K2 relative expression were high, followed by the relative expression of PIP5K8, PIP5K9, and PIP5K6 and PIP5K11 only have fewer expressions. Conclusion It is concluded that PIP5Ks may have a certain effect on the inflorescence axis traits in ramie. The differential expression of PIP5K on the inflorescence axis has the certain regularity with the development of inflorescence.