Analysis on selective extraction effect of six various cyclodextrins on components in chinese patent medicine compound xuezhining / 中草药

ABSTRACT

Objective To analyze the selective extraction effect of six various cyclodextrins (α-cyclodextrin, β-cyclodextrin, methylated-β-cyclodextrin, hydroxypropyl-β-cyclodextrin, sulfobutyl ether-β-cyclodextrin, and carboxymethyl-β-cyclodextrin) on components in Chinese patent medicine Compound Xuezhining (XZN), and to establish functional equation of selective extraction effect of cyclodextrin on different chemical ingredients. Methods The chemical ingredients in each cyclodextrin extract of XZN were detected by ultrahigh-performance liquid chromatography (UPLC) fingerprints, principal component analysis (PCA) was employed to analyze the peak areas of known chemical ingredients in all samples. The structural parameters of the known components in XZN were calculated and inquired using computer aided analysis to analyze the selective extraction effect of various cyclodextrins on components. 1stOpt pro1.5 was applied to establish functional equation of selective extraction effect of cyclodextrin on chemical ingredients with different structures. Results Different extracts of XZN were distinguished effectively via PCA, and most discriminant variables for different extraction methods were obtained simultaneously. Various cyclodextrins have similar trends of selective extraction effect on components, and have different extraction effects for molecule with different properties. The functional equation between the extraction capacity of various cyclodextrins and the structural properties of the constituents was established, and the correlation coefficient is good (r > 0.86). Conclusion Different types of cyclodextrins have a selective extraction effect on the compound in XZN, which provides a theoretical basis for the selection and application of cyclodextrin and its derivatives in traditional Chinese medicine.