Analysis on chemical constituents of Pheretima aspergillum by UPLC-Q-TOF-MS / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 252-262, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-853026
ABSTRACT
Objective:
To establish a method for the analysis of constituents of Pheretima aspergillum by UPLC-Q-TOF-MS.Methods:
The analysis was performed on an Acquity UPLC BEH C18 column (100 mm×2.1 mm, 1.7 μm) by gradient elution. The mobile phase consist of 0.1% formic acid-acetonitrile and 0.1% formic acid-water at a flow rate of 0.3 mL/min. The column temperature was at 35 ℃. The information of the compounds was acquired in positive and negative mode.Results:
Total 83 compounds were inferred, contains 11 free amino acids, 26 organic acids, 11 necleosides, 5 dipeptides and cyclic dipeptideds and other 21 nitrogen-containing substances, the rest on a total of 10.Conclusion:
The method is accurate, rapid, and sensitive, and provide a basis for further clarifying the material basis of its efficacy and the selection of quality control indicators.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2017
Type:
Article
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