Analysis on chemical constituents from Glycyrrhizae Radix et Rhizoma by HPLC-Q-TOF-MS / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 2061-2068, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-853452
ABSTRACT
Objective:
To analyze the main chemical constituents of Glycyrrhizae Radix et Rhizoma by HPLC-Q-TOF-MS in the positive and negative ions mode.Methods:
The chromatographic fingerprint was obtained with Diamonsil II C18 column (250 mm×4.6 mm, 5 μm) and gradient elution with 0.05% H2O-formic acid (A)-acetonitrile (B), and the flow rate was 1 mL/min. The column temperature was maintained at 35℃. The detection wavelength was of 200-600 nm. Positive and negative ions MS information of Bruker Daltonics 1200 HPLC-Q-TOF was coupled with elemental analysis and compared with the literature data to analyze the compounds information.Results:
Combined with the accurate relative molecular mass of compounds provided by HPLC-Q-TOF-MS, 40 compounds were identificated in the ethanolic extract from Glycyrrhizae Radix et Rhizoma, which include 28 flavonoids, 11 triterpenoids saponins, and one coumarin.Conclusion:
The method provides the technical support for the quality control of Glycyrrhizae Radix et Rhizoma, and contributes the reference data to elucidating the potential basis of Glycyrrhizae Radix et Rhizoma
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2016
Type:
Article
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