Study on differential expression of miRNA in serum of rats after renal injury induced by aristolochic acid I / 中草药

ABSTRACT

Objective: To screen the miRNA differential expression profile in serum of rats after renal injury induced by aristolochic acid I, (AAI) to identify the target of miRNA in the mechanism of aristolochic acid nephropathy (AAN), and to make further study on the mechanism of AAN. Methods: Eighteen male SD rats were randomly divided into two groups, control group and AAI group (2.25 mg/kg). Rats were ip injected with AAI once daily for 14 d. On day 14, blood collected was used for the determination of miRNA in serum by miRNA microarray; Renal pathological changes were examined by HE staining. The miRNA that expressed significantly different would be verified by real time quantitative PCR (qPT-PCR). Target genes were predicted using bioinformatics as well as Pathway analysis did. Results: HE staining revealed that kidneys were damaged with different degrees. By significance analysis of microarray based on microarray screening, significantly different expression of five miRNA (miR-10a-3p, miR-207, miR-3594-3p, miR-874-3p, and miR-9a-3p) which were up-regulated were obtained, while there were no miRNAs which were down-regulated. qPT-PCR approved the results as well. It was suggested that 16 genes, such as Rhebl1 and Usp10, might be the target genes. Pathway analysis showed a greater correlation between MAPK signaling pathways. Conclusion: The differential expressed miRNAs obtained by gene analysis might be related with AAI and further studies on the mechanism of some miRNA would be a new target of gene therapy and provide an effective basis for the further studies of bioinformatics.