Pharmacokinetic study of ligustilide, senkyunolide A, and butylphthalide in supercritical extraction fraction from Xiongqiong Decoction in rats / 中草药

ABSTRACT

Objective: To investigate the pharmacokinetics of ligustilide, senkyunolide A, and butylphthalide in supercritical CO2 extraction (SFE) fraction of Xiongqiong Decoction after ig administration in rats. Methods: Plasma samples were collected according to different time periods after ig administration, and the concentration of ligustilide, senkyunolide A, and butylphthalide in plasma samples was determined by HPLC so as to calculate the pharmacokinetic parameters and establish the metabolism curves by PKSolver 2.0 pharmacokinetic software. Results: Excellent liner relationship of ligustilide, senkyunolide A, and butylphthalide was obtained in the range of 0.416-16.640 μg/mL, 0.40-16.05 μg/mL, and 0.52-31.50 μg/mL, respectively. The recovery of the method was more than 84%, and the intra- and inter-day RSD values were less than 5%. The pharmacokinetics parameters of ligustilide were as follows t1/2 (2.082 0 ± 0.637 6) h, Cmax (3.272 5 ± 0.955 5) μg/mL, AUC0-t (15.400 ± 1.812) μg∙h/mL, and the pharmacokinetics parameters of senkyunolide A and butylphthalide were similar to those of ligustilide. Conclusion: The method for the determination of three effective components from Xiongqiong Decoction in plasma samples is precise, specific, and suitable for the pharmacokinetic study and determination of the three effective components simultaneously.