Accumulation of main ginsenoside components in somatic embryogenesis and plantlet regeneration of Panax notoginseng / 中草药
Chinese Traditional and Herbal Drugs
; (24): 2753-2758, 2013.
Article
in Zh
| WPRIM
| ID: wpr-855122
Responsible library:
WPRO
ABSTRACT
Objective:
To investigate the accumulation of total saponins and ginsenosides (Rg1, Re, and Rb1) during somatic embryogenesis and plantlet regeneration in Panax notoginseng.Methods:
The dynamic accumulation of the total saponins and three components of ginsenosides (Rg1, Re, and Rb1) was analyzed by visible spectrophotometry and high-performance liquid chromatography methods, respectively.Results:
The content of total saponins increased from the minimum at embryogenic callus (2.47%) to the maximum at plantlets (7.79%) during somatic embryogenesis development. Because the different specific growth rate of different materials, total saponins accumulation efficiency sort order plantlets > earlier embryos > later embryos > embryogenic callus. We also found that the total content of the three ginsenosides (Rg1, Re, and Rb1) reached the maximum (7.05 mg/g) in the later embryos, and the minimum (2.78 mg/g) was in the embryogenic callus. The contents of ginsenosides Rg1 and Rb1 reached their maximum (2.24 and 4.03 mg/g) respectively in the earlier and later embryos, and the maximum (1.21 mg/g) of ginsenoside Re content was in embryogenic callus. The accumulation (1.947 4 mg/g) of the three ginsenosides minimum occurred in plantlets, and the maximum (5.022 5 mg/g) reached in earlier embryos.Conclusion:
Both total saponin contents and accumulation efficiency reach the maximum in plantlets. Three ginsenoside contents and accumulation reach the maximum in later and earlier embryos, respectively.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Traditional and Herbal Drugs
Year:
2013
Type:
Article