Comparison of the establishment methods of focal cerebral ischemic model in rats / 中国脑血管病杂志

ABSTRACT

Objective: To compare the advantages and disadvantages of the intraluminal suture, electrocautarization of middle cerebral artery combined with unilateral ligation of common carotid artery and electrocautarization of middle cerebral artery combined with bilateral ligation of common carotid artery for establishment of focal cerebral ischemia models in rats. Methods: The focal cerebral ischemia rat models were induced by 3 means. Twenty rats were randomly allocated into intraluminal suture group (n = 6), electrocautarization with unilateral ligation group (n = 7), and electrocautarization with bilateral ligation group (n = 7) according to the different methods for establishment of the rat model. A laser Doppler flow metry was used to detect the relative values of the cerebral blood flow before and after cerebral infarction. The neurological deficit scores of models were measured after 24 h, and the brain slices were stained with 2,3,5-triphenyl tetrazolium chloride. The volume of cerebral infarction was calculated. Results: Circled digit oneThe neurological deficit scores in the intraluminal suture group (9.6 ± 0.6) and the electrocautarization with bilateral ligation group (9.8 ± 0.6) were higher than those in the electrocautarization with unilateral ligation group (5.7 ± 0.7). The differences were statistically significant (P < 0.01). Circled digit twoThe volume of cerebral infarction in the intraluminal suture group (131.5 ± 15.8) mm3 and the electrocautarization with bilateral ligation group (141.7 ± 19.8) mm3 were larger than that in the electrocautarization with unilateral ligation group (39.9 ± 11.4) mm3. The differences were statistically significant (P < 0.01). Circled digit threeThe relative value of cerebral blood flow (CBF) in the electrocautarization with bilateral ligation group was lower than that in the electrocautarization with unilateral ligation group. The difference was statistically significant (P < 0.05). After reperfusion, the relative value of CBF in the intraluminal suture group was higher than that in the electrocautarization with bilateral ligation group. The difference was statistically significant (P < 0.05). Conclusion: The three methods had their advantages and disadvantages in inducing focal cerebral ischemia model in rats. A different model should be used according to the purposes of research.