Fingerprint of serum containing rhubarb bound anthraquinones / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish the fingerprint of the serum containing rhubarb bound anthraquinones, and to analyze the drug-originated constituents in serum containing drugs by serum pharmacochemistry. METHODS: Rat serum containing drugs were prepared after intragastric administration of the extract of rhubarb bound anthraquinones. Fingerprints of 10 batches serum containing drugs were determined by HPLC, and the common mode was established. Comparing the chromatogram of serum containing drugs with the one of blank serum, the common peaks of drug-originated constituents in blood were identified. Comparing the retention time and spectrum of the chromatographic peaks of serum containing drugs with the ones of extract, the sources of drug-originated constituents were analyzed. Comparing the retention time and spectrum of the chromatographic peaks of serum containing drugs with the ones of mixed reference substances, some drug-originated constituents were identified. RESULTS: There are 20 common peaks of drug-originated constituents in 10 batches serum containing drugs, 14 of them were in vitro prototype, and the other 6 were metabolites in vivo. Thirteen out of 14 prototype constituents were identified as aloe emodin-8-O-glucopyranoside, rhein-8-O-glucopyranoside, emodin-1-O-glucopyranoside, chrysophanol-1-O-glucopyranoside, chrysophanol-8-O-glucopyranoside, emodin-8-O-glucopyranoside, aloe emodin-3-CH2-O-glucopyranoside, physcion-8-O-glucopyranoside, aloe emodin, rhein, emodin, chrysophanol, and physcion, respectively. Other 1 prototype constituent and 6 metabolites possessed the same UV absorption character as that of anthraquinones. CONCLUSION: The method established in this study is accurate and feasible, and can be used for the analysis of the drug-originated constituents in serum containing rhubarb bound anthraquinones.