Physical Stability Evaluation of Glycyrrhetinic Acid Derivatives-Mediated Liposomes / 中国药学杂志

ABSTRACT

OBJECTIVE: To evaluate the physical stability of glycyrrhetinic acid derivatives-mediated coumarin 6(Cou6) liposomes and confirm the applicability of different stability tests on liposomes. METHODS: Film dispersion-ultrasonic method was used to prepare Cou6 liposomes, PEG-modified liposomes and glycyrrhetinic acid-mediated liposomes. The stability constants, membrane stability, serum stability and leakages of the six kinds of liposomes were studied. RESULTS: The physical stability of the liposomes without modification was poor. As for the glycyrrhetinic acid-mediated liposomes, the stability constants at 15 min were 5.37-7.32 and the concentrations of Triton X-100 were 0.207‰-0.380‰ when half liposome membranes were destroyed. The serum stability in 24 h and leakages in 7 or 14 d showed good stability with little change. CONCLUSION: The physical stability is one of the key pharmaceutical properties of liposomes. The stability constant, serum stability and leakage tests and the method of membrane stability we have established can be used to study the stability of liposomes.