Reduction of iron deposition by verbascoside in HO-1 overexpression rats astrocytes / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the effect of verbascoside on iron deposition of astrocyte cell overexpressing heme oxygenase-1 gene, and to seek new drug in treating Alzheimer's disease. METHODS: Primary cultured astrocyte cells of rats were cultured and purified in vitro. With gene recombination and transfer techniques, transfect HO-1 gene into primary cultured astrocyte cells. HO-1 transfected astrocyte were then treated by different concentrations of verbascoside. Proliferation of cell was measured by CCK-8 assay and expression of HO-1 protein was analyzed by Western blotting. Iron deposition was also visualized by Perl's Prussian blue stain. RESULTS: Immunofluorescence staining showed that most of the cells were GFAP-postitive and the purity was over 95%. The expression of HO-1 protein was increased in HO-1 injury model of astrocyte. Treatment of verbascoside with variable concentration caused the decrease of HO-1 protein expression more or less and reduction of iron deposition in transfected cells(20 μmol·L-1 reducing 47.69%, 40 μmol·L-1 reducing 51.63%). CONCLUSION: Verbascoside could enhance astrocyte cell resistance against injury induced by overexpression of heme oxygenase-1 gene.