A comparative study of protective action on endotheliocytes of fucoidan with different molecular weight and its mechanism / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the protective action on endotheliocytes of fucoidan with different molecular weight from Laminaria japonica, and to explore its mechanism. METHODS: In vivo, endotheliocytes injury model in rats was used to examine the protective action on endotheliocytes of fucoidan with different molecular weight from Laminaria japonica; aortal endothelium section by HE stain under optical microscope was observed, and the the content of von willebrand factor (vWF) in plasma was determined by ELISA. In vitro, endothelial cell culture was used to determine the effect of fucoidan on content of vWF and endothelial microparticles (EMPs). RESULTS: In vivo, aortal endothelium section by HE stain under optical microscope showed low molecular weight (LMW) fucoidan had a remarkable protective effect, and the protection effect of LMWF fucoidans are stronger than middle molecular weight (MMW) fu-coidans. Administration of LMW fucoidan significantly decreased the vWF content. Administration of MMW fucoidan didn't change vWF content. In vitro, Administration of LMW fucoidan significantly decreased the vWF content and EMPs numbers. Administration of MMW fucoidan didn't change the vWF content and EMPs numbers. CONCLUSION: The LMW fucoidan fractions have good protective action on endothelial cells. The protective effect of LMW fucoidans is significantly stronger than MMW fucoidans. The protective action on endotheliocytes of LMW fucoidans relies on decreasing the concentration of EMPs and vWF, and thus inhibiting platelet activation indirectly.