Effect of MiR-760 on Colon Cancer Metastasis by Inhibiting the Key Molecules of Calcium Ion Signal / 胃肠病学

ABSTRACT

Background: MicroRNAs (miRNAs) are considered as new biomarkers related to cancer treatment. Studies have shown that miR-760 plays an important role in the development of colorectal cancer. Aims: To investigate the regulatory effect and mechanism of miR-760 on proliferation, cycle, migration and invasion of colon cancer cells. Methods: qRT-PCR was used to detect the expression of miR-760 in colon cancer tissue and colon cancer cell lines HCT116, HT29, SW480 and SW620. The targeted relationship between miR-760 and STIM1 was verified by dual luciferase reporter gene test. Expression of STIM1 in colon cancer cells and tissue was detected by Western blotting and immunohistochemistry, respectively. CCK-8 assay was used to detect cell proliferation ability, flow cytometry was used to detect cell cycle, and Transwell assay was used to detect cell migration and invasion. The xenograft model of colon cancer in nude mice was constructed, and the effect of miR-760 on tumor growth was measured. Results: Expression of miR-760 was significantly decreased in colon cancer tissue and colon cancer cells, while expression of STIM1 was significantly increased in colon cancer tissue and colon cancer cells. MiR-760 inhibited expression of STIM1 by complementary binding to the 3'-UTR of STIM1. Overexpression of miR-760 inhibited colon cancer cell proliferation, S-phase arrest, migration and invasion, and up-regulation of STIM1 reversed the inhibitory effect. In the xenograft model of colon cancer in nude mice, overexpression of miR-760 down-regulated expression of STIM1 and inhibited tumor growth. Conclusions: MiR-760 is involved in the regulation of biological behavior of colon cancer cells by targeting STIM1, and plays a role in tumor inhibition of colon cancer.