In vivo MRI tracking after transplantation of PEG/PEI-SPIO labeled adipose derived stem cells in AD rats models / 中国介入影像与治疗学

ABSTRACT

Objective: To explore the feasibility of tracking the transplantation of adipose derived stem cells (ADSC) for Alzheimer disease (AD) with polyethylene glycol/polyethyleneimine modified superparamagnetic iron oxide (PEG/PEI-SPIO) with in vivo MRI. Methods: Totally 48 AD rat models were randomly divided into labeled group, unlabeled group and phosphate buffer saline (PBS) group (each n=16). PEG/PEI-SPIO labeled or unlabeled ADSC and PBS were injected into the ventricle of rats, respectively. Other 16 rats were taken as the sham operation group. On the 7th, 14th, 21st and 28th day after transplantation, T2 value of temporal parietal cortex, hippocampus and cerebellum were measured with cranial MRI, and the iron content of brain tissue were measured on the above-mentioned brain areas. Results: T2 values of the hippocampus and the cortex were shorter in labeled group than in other groups after transplantation (all P<0.05), and of the cortex were shorter than of the hippocampus and cerebella on the 7th and 14th day, of the hippocampus were shorter than of cortex and cerebella on the 21st and 28th day in the labeled group (all P<0.05). At the same time point, the iron content of the labeled group was higher than that of the other groups in each brain region (all P<0.05). The iron content of the cortex was higher than of other regions in the labeled group on the 7th and 14th day (all P<0.05), and the iron content of the hippocampus was higher than of other regions in the labeled group on the 21st and 28th day (all P<0.05). Conclusion: Being labeled with PEG/PEI-SPIO, the distribution and migration of ADSC in the brain of rat AD models could be tracked with in vivo MRI.