Cell cycling status of human cord blood CD34+ cells during ex vivo expansion is related to the level of very late antigen expression
Journal of Korean Medical Science
; : 20-24, 2001.
Article
in En
| WPRIM
| ID: wpr-86344
Responsible library:
WPRO
ABSTRACT
Very late antigen-4 (VLA-4), which binds to the extracellular matrix protein fibronectin, is an integrin molecule known to be modulated during mobilization of CD34+ cells, and to be involved in signaling the mobilization stimuli. On the hypothesis that cell cycling status might be different depending on the level of VLA-4 expression, we investigated the DNA contents of human cord blood CD34+ cells during ex vivo expansion by recombinant human thrombopoietin and flt3-ligand with simultaneous measurement of surface VLA-4 at the 1st and 4th week. During this ex vivo expansion, expression of VLA-4 increased and almost all cells became VLA-4+ until the 4th day of culture. Expression of VLA-4 was maintained in the major population of the cultured cells until the 4th week. The cells in S/G2/M phase were greater in number in VLA-4 high fraction than in VLA-4 low fraction (n=4, p<.001). Furthermore, the fraction of cells in S/G2/M phase increased as the expression of VLA-4 became higher. These results suggest that cord blood CD34+ cells expressing high levels of VLA-4 have more proliferative activities.
Key words
Full text:
1
Index:
WPRIM
Main subject:
DNA
/
Hematopoietic Stem Cells
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Integrins
/
Cells, Cultured
/
G2 Phase
/
Immunophenotyping
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Receptors, Lymphocyte Homing
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S Phase
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Antigens, CD34
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Fetal Blood
Limits:
Humans
/
Newborn
Language:
En
Journal:
Journal of Korean Medical Science
Year:
2001
Type:
Article