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In vitro development of canine somatic cell nuclear transfer embryos in different culture media
Journal of Veterinary Science ; : 233-235, 2015.
Article in English | WPRIM | ID: wpr-86394
ABSTRACT
The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 sequential media. Our results showed that the G1/G2 media yielded significantly higher morula and blastocyst development in canine SCNT embryos (26.1% and 7.8%, respectively) compared to PZM-3 (8.5% and 0%) or mSOF (2.3% and 0%) media. In conclusion, this study suggests that blastocysts can be produced more efficiently using G1/G2 media to culture canine SCNT embryos.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Blastocyst / Cloning, Organism / Culture Media / Embryo Culture Techniques / Embryonic Development / Dogs / Nuclear Transfer Techniques Limits: Animals Language: English Journal: Journal of Veterinary Science Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Blastocyst / Cloning, Organism / Culture Media / Embryo Culture Techniques / Embryonic Development / Dogs / Nuclear Transfer Techniques Limits: Animals Language: English Journal: Journal of Veterinary Science Year: 2015 Type: Article