Effect of Ru′ai Shuhou Prescription on Proliferation and Metastasis of Breast Cancer MDA-MB-453 Cells Through SDF-1/CXCR4 Biological Axis / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae
; (24): 106-112, 2020.
Article
in Zh
| WPRIM
| ID: wpr-872705
Responsible library:
WPRO
ABSTRACT
Objective:
To investigate the effect of Ru′ai Shuhou prescription (RSR) drug-containing serum on the proliferation and invasion ability of breast cancer cells MDA-MB-453 based on the biological axis of stromal cell-derived factor-1(SDF-1)/chemokine receptor 4 (CXCR4).Method:
A model of MDA-MB-453 cells with SDF-1-induced high expression of CXCR4 was established, and the rat drug-serum containing RSR and blank rat serum were prepared respectively. The cells were divided into fetal bovine serum control group (Blank), blank rat serum group, SDF-1+blank rat serum group, SDF-1+RSR group, AMD3100+ SDF-1+blank rat serum group, and AMD3100+ SDF-1+RSR group. After intervention for 48 h, cell proliferation was detected by cell counting kit-8 (CCK-8) assay, cell invasion ability was detected by transwell assay, and mRNA and protein expressions of CXCR4, matrix metalloproteinase-2 (MMP-2) and MMP-9 were detected by Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR) and Western blot, respectively.Result:
As compared with the blank serum group, the proliferation of MDA-MB-453 cells was promoted and expression of CXCR4 mRNA was increased significantly when SDF-1 was 100 μg·L-1 (P<0.05). As compared with SDF-1+blank rat serum group, RSR inhibited the proliferation and invasion of MDA-MB-453 cells induced by SDF-1, and at the same time, down-regulated the mRNA and protein expressions of CXCR4, MMP-2 and MMP-9 (P<0.05). After pre-treatment with AMD3100 for 24 h, the inhibitory effect of RSR to cell proliferation was significantly increased (P<0.05), and meanwhile, the decreases in mRNA and protein expression of CXCR4, MMP-2 and MMP-9 were more obvious, with statistically significant differences (P<0.05).Conclusion:
Through SDF-1/CXCR4 biological axis, RSR could down-regulate the expression of MMP-2 and MMP-9, reduce the degradation of extracellular matrix (ECM), and then inhibit the metastasis of MDA-MB-453 cells. In addition, it has a synergistic effect with CXCR4 inhibitor AMD3100.
Full text:
1
Index:
WPRIM
Type of study:
Prognostic_studies
Language:
Zh
Journal:
Chinese Journal of Experimental Traditional Medical Formulae
Year:
2020
Type:
Article