Expression and regulation of XBP1 in mouse uterus during early pregnancy / 生理学报
Acta Physiologica Sinica
;
(6): 208-216, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-878249
ABSTRACT
The transcription factor X-box binding protein-1 (XBP1) plays a key role in unfolded protein reaction. This study was aimed to investigate the expression pattern and regulation of XBP1 in the mouse uterus during early pregnancy. The methods of immunohistochemistry (IHC) and real time quantitative RT-PCR were used to test XBP1 expression in early pregnancy, artificial decidualization, oestrous cycle and hormone-regulated mouse models. The results showed that XBP1 was spatiotemporally expressed in mouse uterus during early pregnancy. The XBP1 protein was mainly detected in the luminal and glandular epithelia on days 1-4 of pregnancy, and was strongly detected in the decidual area on days 5-8 of pregnancy. Similarly, XBP1 expression was also mainly expressed in decidual cells following artificial decidualization. During the oestrous cycle, Xbp1, Xbp1u, and Xbp1s mRNA was predominantly present in proestrus. In the ovariectomized uterus, the expression of XBP1 in luminal and glandular epithelia was up-regulated after estrogen treatment. These results suggest that XBP1 is associated with embryo implantation and decidualization during early pregnancy in mice, and the expression of XBP1 in luminal and glandular epithelia may be regulated by estrogen.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Embryo Implantation
/
Uterus
/
RNA, Messenger
/
Decidua
/
Estrogens
Type of study:
Prognostic study
Limits:
Animals
/
Pregnancy
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2021
Type:
Article
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