MicroRNA-135a in ABCA1-labeled Exosome is a Serum Biomarker Candidate for Alzheimer's Disease / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 19-28, 2021.
Article
in En
| WPRIM
| ID: wpr-878317
Responsible library:
WPRO
ABSTRACT
Objective@#In the present study, the ABCA1 was used as a label to capture specific exosomes, the level of ABCA1-labeled exosomal microRNA-135a (miR-135a) was evaluated for the diagnosis of Alzheimer's disease (AD), especially in patients with early stages of AD.@*Methods@#This is a preliminary research focused on the levels of ABCA1 in WBCs, RBCs, HT-22 cells, and neuron cells. The diagnostic value of ABCA1-labeled exosomal miR-135a was examined using the CSF and serum of APP/PS1 double transgenic mice, and 152 patients with SCD, 131 patients with MCI, 198 patients with DAT, and 30 control subjects.@*Results@#The level of ABCA1 exosomes harvested from HT-22 cells and neuron culture medium was significantly higher compared to that of RBCs and WBCs ( @*Conclusion@#This study outlines a method to capture specific exosomes and detect them using immunological methods, which is more efficient for early diagnosis of AD.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Mice, Transgenic
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Biomarkers
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Cell Line
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MicroRNAs
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Erythrocytes
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Exosomes
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Alzheimer Disease
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Cognitive Dysfunction
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ATP Binding Cassette Transporter 1
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Leukocytes
Type of study:
Screening_studies
Limits:
Aged80
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Animals
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Female
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Humans
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Male
Language:
En
Journal:
Biomedical and Environmental Sciences
Year:
2021
Type:
Article