RT-nPCR Assays for Amplification and Sequencing of VP1 Genes in Human Enterovirus A-D from Clinical Specimens / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 829-838, 2020.
Article
in En
| WPRIM
| ID: wpr-878347
Responsible library:
WPRO
ABSTRACT
Objective@#To develop RT-nPCR assays for amplifying partial and complete VP1 genes of human enteroviruses (HEVs) from clinical samples and to contribute to etiological surveillance of HEV-related diseases.@*Methods@#A panel of RT-nPCR assays, consisting of published combined primer pairs for VP1 genes of HEV A-C and in-house designed primers for HEV-D, was established in this study. The sensitivity of each RT-nPCR assay was evaluated with serially diluted virus stocks of five serotypes expressed as CCID @*Results@#The sensitivity of RT-nPCR assays for amplifying partial VP1 gene of HEVs was 0.1 CCID @*Conclusion@#This RT-nPCR system is capable of amplifying the partial and complete VP1 gene of HEV A-D, providing rapid, sensitive, and reliable options for molecular typing and molecular epidemiology of HEVs in clinical specimens.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Molecular Epidemiology
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Enterovirus B, Human
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Reverse Transcriptase Polymerase Chain Reaction
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Enterovirus A, Human
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Enterovirus C, Human
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Enterovirus D, Human
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Capsid Proteins
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Molecular Typing
Limits:
Humans
Language:
En
Journal:
Biomedical and Environmental Sciences
Year:
2020
Type:
Article