LMP2-DC Vaccine Elicits Specific EBV-LMP2 Response to Effectively Improve Immunotherapy in Patients with Nasopharyngeal Cancer / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 849-856, 2020.
Article
in En
| WPRIM
| ID: wpr-878349
Responsible library:
WPRO
ABSTRACT
Objective@#To evaluate the safety and effectiveness of a vaccine based on latent membrane protein 2 (LMP2) modified dendritic cells (DCs) that boosts specific responses of cytotoxic T lymphocytes (CTLs) to LMP2 before and after intradermal injection in patients with nasopharyngeal carcinoma (NPC).@*Methods@#DCs were derived from peripheral blood monocytes of patients with NPC. We prepared LMP2-DCs infected by recombinant adenovirus vector expressing LMP2 (rAd-LMP2). NPC patients were immunized with 2 × 10 @*Results@#We demonstrated that DCs derived from monocytes displayed typical DC morphologies; the expression of LMP2 in the LMP2-DCs vaccine was confirmed by immunocytochemical assay. Twenty-nine patients with NPC were enrolled in this clinical trial. The LMP2-DCs vaccine was well tolerated in all of the patients. Boosted responses to LMP2 peptide sub-pools were observed in 18 of the 29 patients with NPC. The follow-up data of 29 immunized patients from April, 2010 to April 2015 indicated a five-year survival rate of 94.4% in responders and 45.5% in non-responders.@*Conclusion@#In this pilot study, we demonstrated that the LMP2-DCs vaccine is safe and effective in patients with NPC. Specific CTLs responses to LMP2 play a certain role in controlling and preventing the recurrence and metastasis of NPC, which warrants further clinical testing.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Dendritic Cells
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Injections, Intradermal
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T-Lymphocytes, Cytotoxic
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China
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Viral Matrix Proteins
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Nasopharyngeal Neoplasms
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Cancer Vaccines
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Nasopharyngeal Carcinoma
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Immunotherapy
Type of study:
Clinical_trials
Limits:
Adult
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Aged
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Female
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Humans
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Male
Country/Region as subject:
Asia
Language:
En
Journal:
Biomedical and Environmental Sciences
Year:
2020
Type:
Article