Light and carbon dioxide-driven synthesis of high-density fuel in Synechococcus elongates UTEX 2973 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 2126-2138, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-878472
ABSTRACT
Development of "liquid sunshine" could be a key technology to deal with the issue of fossil fuel depletion. β-caryophyllene is a terpene compound with high energy density and has attracted attention for its potential application as a jet fuel. The high temperature and high light-tolerant photosynthetic cyanobacterium Synechococcus elongatus UTEX 2973 (hereafter Synechococcus 2973), whose doubling time is as short as 1.5 h, has great potential for synthesizing β-caryophyllene using sunlight and CO₂. In this study, a production of ~121.22 μg/L β-caryophyllene was achieved at 96 h via a combined strategy of pathway construction, key enzyme optimization and precursor supply enhancement. In addition, a final production of ~212.37 μg/L at 96 h was realized in a high-density cultivation. To our knowledge, this is the highest production reported for β-caryophyllene using cyanobacterial chassis and our study provide important basis for high-density fuel synthesis in cyanobacteria.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Photosynthesis
/
Carbon Dioxide
/
Synechococcus
/
Biofuels
/
Light
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2020
Type:
Article
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