Propeptide-mediated protein folding: mechanism and its impact on lipase / 生物工程学报
Chinese Journal of Biotechnology
; (12): 88-99, 2021.
Article
in Zh
| WPRIM
| ID: wpr-878545
Responsible library:
WPRO
ABSTRACT
The formation of most proteins consists of two steps:
the synthesis of precursor proteins and the synthesis of functional proteins. In these processes, propeptides play important roles in assisting protein folding or inhibiting its activity. As an important polypeptide chain coded by a gene sequence in lipase gene, propeptide usually functions as an intramolecular chaperone, assisting enzyme molecule folding. Meanwhile, some specific sites on propeptide such as glycosylated sites, have important effect on the activity, stability in extreme environment, methanol resistance and the substrate specificity of the lipase. Studying the mechanism of propeptide-mediated protein folding, as well as the influence of propeptide on lipases, will allow to regulate lipase by alternating the propeptide folding behavior and in turn pave new ways for protein engineering research.Key words
Full text:
1
Index:
WPRIM
Main subject:
Protein Precursors
/
Substrate Specificity
/
Protein Folding
/
Molecular Chaperones
/
Lipase
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2021
Type:
Article