3D organization profiling of human hepatocellular carcinoma cell line PLC/PRF/5 in comparison with normal human liver cell line L02 by in situ Hi-C / 生物工程学报
Chinese Journal of Biotechnology
; (12): 331-341, 2021.
Article
in Zh
| WPRIM
| ID: wpr-878566
Responsible library:
WPRO
ABSTRACT
Genetic and epigenetic alterations accumulate in the process of hepatocellular carcinogenesis, but the role of genomic spatial organization in HCC is still unknown. Here, we performed in situ Hi-C in HCC cell line PLC/PRF/5 compared with normal liver cell line L02, together with RNA-seq and ChIP-seq of SMC3/CTCF/H3K27ac. The results indicate that there were significant compartment switching, TAD shifting and loop pattern altering in PLC/PRF/5. These spatial changes are correlated with abnormal gene expression and more opening promoter regions of the HCC cell line. Thus, the 3D genome organization alterations in PLC/PRF/5 are important in epigenetic mechanisms of HCC tumorigenesis.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Cell Line
/
Carcinoma, Hepatocellular
/
Genomics
/
Cell Line, Tumor
/
Liver Neoplasms
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2021
Type:
Article