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Analysis and comparison of metabolic processes of salvianolic acid A and salvianolic acid B in rats based on UHPLC-LTQ-Orbitrap MS technology / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 2276-2286, 2021.
Article in Chinese | WPRIM | ID: wpr-879188
ABSTRACT
The metabolites of salvianolic acid A and salvianolic acid B in rats were analyzed and compared by ultra-high-perfor-mance liquid chromatography with linear ion trap-orbitrap mass spectrometry(UHPLC-LTQ-Orbitrap MS). After the rats were administrated by gavage, plasma at different time points and urine within 24 hours were collected to be treated by solid phase extraction(SPE), then they were gradient eluted by Acquity UPLC BEH C_(18) column(2.1 mm×100 mm, 1.7 μm) and 0.1% formic acid solution(A)-acetonitrile(B) mobile phase system, and finally all biological samples of rats were analyzed under negative ion scanning mode. By obtaining the accurate relative molecular mass and multi-level mass spectrometry information of metabolites, combined with the characteristic cleavage law of the reference standard and literature reports, a total of 30 metabolites, including salvianolic acid A and B, were identified. Among them, there were 24 metabolites derived from salvianolic acid A, with the main metabolic pathways including ester bond cleavage, dehydroxylation, decarboxylation, hydrogenation, methylation, hydroxylation, sulfonation, glucuronidation, and their multiple reactions. There were 15 metabolites of salvianolic acid B, and the main biotransformation pathways were five-membered ring cracking, ester bond cleavage, decarboxylation, dehydroxylation, hydrogenation, methylation, sulfonation, glucuronidation, and their compound reactions. In this study, the cross-metabolic profile of salvianolic acid A and B was elucidated completely, which would provide reference for further studies on the basis of pharmacodynamic substances and the exploration of pharmacological mechanism.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Mass Spectrometry / Technology / Benzofurans / Caffeic Acids / Chromatography, High Pressure Liquid / Lactates Type of study: Prognostic study Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Mass Spectrometry / Technology / Benzofurans / Caffeic Acids / Chromatography, High Pressure Liquid / Lactates Type of study: Prognostic study Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2021 Type: Article