Molecular genetic analysis of two individuals with weak D variant of the Rh blood type / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 492-495, 2021.
Article
in Zh
| WPRIM
| ID: wpr-879611
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To explore the molecular basis of two individuals with weak D variant of the Rh blood type.@*METHODS@#Routine serological testing was carried out to detect the D, C, c, E and e antigens of the Rh blood group. The D antigen was further detected with an indirect antiglobulin test. The presence of Rhesus box was detected by PCR to determine the homozygosity of the RHD gene.@*RESULTS@#Both samples were determined as weak D phenotype by the indirect antiglobulin test. DNA sequencing revealed that case 1 harbored a heterozygous 208C>T variant in exon 2 and a heterozygous 1227G>A variant in exon 9; while case 2 harbored homozygous 779A>G variants of exon 5 of the RHD gene. Case 1 was determined as RHD+/RHD+, while case 2 was determined as RHD+/RHD-. The two samples were respectively named as weak D type 122 and weak D type 149 based on the rules of Rhesus Base Nomenclature.@*CONCLUSION@#D negative blood donors should subject to indirect antiglobulin testing and molecular analysis for safer transfusion.
Full text:
1
Index:
WPRIM
Main subject:
Phenotype
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Rh-Hr Blood-Group System
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Blood Donors
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Blood Grouping and Crossmatching
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Alleles
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Genotype
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Molecular Biology
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Medical Genetics
Year:
2021
Type:
Article