Oligomycin A promotes radioresistance in HT29 colorectal cancer cells and its mechanisms / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 113-120, 2021.
Article
in En
| WPRIM
| ID: wpr-880631
Responsible library:
WPRO
ABSTRACT
OBJECTIVES@#Radiotherapy is one of the main therapies for colorectal cancer, but radioresistance often leads to radiotherapy failure. To improve the radioresistance, we explore the effect of oligomycin A, the H@*METHODS@#The effects of different concentrations of oligomycin A on the survival rate and glycolysis of HT29 colorectal cancer cells at different time points were investigated via MTT and glycolysis assay. siRNA-PFK1 was synthesized in vitro and transfected into HT29 cells. The effects of oligomycin A on radiosensitivity of HT29 colorectal cancer cells were measured via MTT and colony formation assay. Western blotting was used to detect the effect of oligomycin A on the expression of glycolytic enzyme PFK1. We compared difference between the effects of siRNA-PFK1 group and oligomycin A combined with siRNA-PFK1 group on cell survival and glycolysis. After 4 Gy X-ray irradiation, the effects of cell survival and glycolysis between the siRNA-PFK1 group and the oligomycin A combined with siRNA-PFK1 group were compared.@*RESULTS@#Compared with the 0 μmol/L oligomycin A group, the cell survival rate of HT29 cells treated with 4 μmol/L oligomycin A was significantly increased (@*CONCLUSIONS@#Oligomycin A can promote the radioresistance of HT29 colorectal cancer cells, which may be related to up-regulation of the PFK1 expression and increase of cell glycolysis.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Oligomycins
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Radiation Tolerance
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Colorectal Neoplasms
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HT29 Cells
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Cell Line, Tumor
Limits:
Humans
Language:
En
Journal:
Journal of Central South University(Medical Sciences)
Year:
2021
Type:
Article