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Generation and phenotypic characterization of S100A9 gene knockout mice by CRISPR/Cas9-mediated gene targeting / 生理学报
Acta Physiologica Sinica ; (6): 482-490, 2021.
Article in Zh | WPRIM | ID: wpr-887683
Responsible library: WPRO
ABSTRACT
S100 calcium binding protein A9 (S100A9) is involved in a variety of biological processes such as inflammation and tumor cell migration and invasion regulation. The purpose of this study was to construct S100A9 gene-edited mice by using CRISPR/Cas9 technology, thereby providing an animal model for exploring the biological functions of this gene. According to the S100A9 gene sequence, the single-stranded small guide RNA (sgRNA) targeting exons 2 and 3 was transcribed in vitro, and a mixture of Cas9 mRNA and candidate sgRNA was injected into mouse fertilized eggs by microinjection. Early embryos were obtained and transferred to surrogate mice, and F
Subject(s)
Full text: 1 Index: WPRIM Main subject: Phenotype / Bronchoalveolar Lavage Fluid / Ovalbumin / Mice, Knockout / Gene Targeting / Calgranulin B / Disease Models, Animal / Gene Knockout Techniques / CRISPR-Cas Systems / Lung Limits: Animals Language: Zh Journal: Acta Physiologica Sinica Year: 2021 Type: Article
Full text: 1 Index: WPRIM Main subject: Phenotype / Bronchoalveolar Lavage Fluid / Ovalbumin / Mice, Knockout / Gene Targeting / Calgranulin B / Disease Models, Animal / Gene Knockout Techniques / CRISPR-Cas Systems / Lung Limits: Animals Language: Zh Journal: Acta Physiologica Sinica Year: 2021 Type: Article