Generation and phenotypic characterization of S100A9 gene knockout mice by CRISPR/Cas9-mediated gene targeting / 生理学报
Acta Physiologica Sinica
; (6): 482-490, 2021.
Article
in Zh
| WPRIM
| ID: wpr-887683
Responsible library:
WPRO
ABSTRACT
S100 calcium binding protein A9 (S100A9) is involved in a variety of biological processes such as inflammation and tumor cell migration and invasion regulation. The purpose of this study was to construct S100A9 gene-edited mice by using CRISPR/Cas9 technology, thereby providing an animal model for exploring the biological functions of this gene. According to the S100A9 gene sequence, the single-stranded small guide RNA (sgRNA) targeting exons 2 and 3 was transcribed in vitro, and a mixture of Cas9 mRNA and candidate sgRNA was injected into mouse fertilized eggs by microinjection. Early embryos were obtained and transferred to surrogate mice, and F
Full text:
1
Index:
WPRIM
Main subject:
Phenotype
/
Bronchoalveolar Lavage Fluid
/
Ovalbumin
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Mice, Knockout
/
Gene Targeting
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Calgranulin B
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Disease Models, Animal
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Gene Knockout Techniques
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CRISPR-Cas Systems
/
Lung
Limits:
Animals
Language:
Zh
Journal:
Acta Physiologica Sinica
Year:
2021
Type:
Article